Mr. Twig was introduced as Herbert Garrison's Teaching Assistant at the end of the episode "Summer Sucks", when Garrison couldn't find Mr. Hat. He wasn't very popular with the students, who continually asked when Mr. Hat was going to come back. He continued being the teaching assistant until the episode "Chef Aid", where someone/something was trying to assassinate Mr. Twig.
After getting busted out of jail by Mr. Hat, Mr. Garrison had to choose between Mr. Hat or Mr. Twig. In his only speaking role, Mr. Twig goes on to tell Mr. Garrison to "Run to him.", him being Mr. Hat. He then says in a French accent, "Love isn't a decision, it's a feeling. If we could decide who we love, it would be much simpler, but much less magical." Mr. Garrison then discards Mr. Twig and he is never seen or mentioned again.
The Stick of Truth
Mr. Twig can be found in the sewers, as a junk item. His description jokingly says he's out of work. He has no other role in the game and thus cannot be befriended unlike Mr. Hat.
Mr. Twig is basically a twig that wears a purple shirt with a pink triangle symbol.
- Mr. Twig's shirt features a symbol, which has been historically used to represent homosexuality. It was used in Nazi concentration camp badges to identify male prisoners who were sent there because of their homosexuality. The badge worn by prisoners was a downward pointing pink triangle. Today, the pink triangle symbol has been reclaimed, it is used inverted as an international symbol of the gay pride and gay rights movement. The use of this symbol on Mr. Twig's shirt, is most likely a reference to Mr. Garrison's closet homosexuality as seen throughout Season Two.